Protein dynamics revealed by NMR relaxation methods

Substrate Modulated Dynamics of the ADP/ATP Transporter Revealed by NMR Relaxation Dispersion

Redox-controlled backbone dynamics of human cytochrome c revealed by 15N NMR relaxation measurements.

Redox-controlled backbone dynamics in cytochrome c (Cyt c) were revealed by 2D 15N NMR relaxation experiments. 15N T1 and T2 values and 1H-15N NOEs of uniformly 15N-labeled reduced and oxidized Cyt c were measured, and the generalized order parameters (S2), the effective correlation time for internal motion (taue), the 15N exchange broadening contributions (Rex) for each residue, and the overal...

Redox-controlled Backbone Dynamics of Human Cytochrome c Revealed by N NMR Relaxation Measurements

Redox-controlled backbone dynamics in cytochrome c (Cyt c) were revealed by 2D N NMR relaxation experiments. N T1 and T2 values and H-N NOEs of uniformly N-labeled reduced and oxidized Cyt c were measured, and the generalized order parameters (S), the effective correlation time for internal motion (τe), the N exchalnge broadening contributions (Rex) for each residue, and the overal correlation ...

Global Dynamics and Exchange Kinetics of a Protein on the Surface of Nanoparticles Revealed by Relaxation-Based Solution NMR Spectroscopy.

The global motions and exchange kinetics of a model protein, ubiquitin, bound to the surface of negatively charged lipid-based nanoparticles (liposomes) are derived from combined analysis of exchange lifetime broadening arising from binding to nanoparticles of differing size. The relative contributions of residence time and rotational tumbling to the total effective correlation time of the boun...

A theory of protein dynamics to predict NMR relaxation.

We present a theoretical, site-specific, approach to predict protein subunit correlation times, as measured by NMR experiments of (1)H-(15)N nuclear Overhauser effect, spin-lattice relaxation, and spin-spin relaxation. Molecular dynamics simulations are input to our equation of motion for protein dynamics, which is solved analytically to produce the eigenvalues and the eigenvectors that specify...